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Servicebio Inc citrate antigen retrieval buffer
Citrate Antigen Retrieval Buffer, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antigen+retrieval+citrate+buffer/antigen+retrieval/pmc13050104-93-11-16
Average 86 stars, based on 1 article reviews
citrate antigen retrieval buffer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Incubation:

Article Title: Exosomes derived from human umbilical cord mesenchymal stem cells alleviate Parkinson's disease and neuronal damage through inhibition of microglia.
Article Snippet: After adding the TH primary antibody and then the secondary antibody, FITC-TSA (Servicebio) was added, and the sections were incubated in the dark for 10 minutes. .. After antigen retrieval with citrate buffer (Servicebio), sections were incubated with Iba-1 primary antibody at 4°C for 12 hours and Cy3-conjugated secondary antibody (donkey, 1:250; Servicebio, Cat# GB21403, RRID: AB_2818951) in the dark at room temperature for 50 minutes, and DAPI (Invitrogen, Carlsbad, CA, USA) was used to stain the nuclei. .. The number of Iba-1-positive microglia was automatically determined using a fluorescent microscope (Nikon, Tokyo, Japan) with the NIS Elements Software (version 4.2, Nikon), and the defined area contained the entire SN on both sides (Wang et al., 2020).

Staining:

Article Title: Exosomes derived from human umbilical cord mesenchymal stem cells alleviate Parkinson's disease and neuronal damage through inhibition of microglia.
Article Snippet: After adding the TH primary antibody and then the secondary antibody, FITC-TSA (Servicebio) was added, and the sections were incubated in the dark for 10 minutes. .. After antigen retrieval with citrate buffer (Servicebio), sections were incubated with Iba-1 primary antibody at 4°C for 12 hours and Cy3-conjugated secondary antibody (donkey, 1:250; Servicebio, Cat# GB21403, RRID: AB_2818951) in the dark at room temperature for 50 minutes, and DAPI (Invitrogen, Carlsbad, CA, USA) was used to stain the nuclei. .. The number of Iba-1-positive microglia was automatically determined using a fluorescent microscope (Nikon, Tokyo, Japan) with the NIS Elements Software (version 4.2, Nikon), and the defined area contained the entire SN on both sides (Wang et al., 2020).

Article Title: Discovery of a potent Gilteritinib-based FLT3-PROTAC degrader for the treatment of Acute myeloid leukemia.
Article Snippet: Fms-like tyrosine receptor kinase 3 (FLT3) proteolysis targeting chimeras (PROTACs) emerge as a promising approach to overcome the limitations of FLT3 inhibitors, while the development of orally bioavailable FLT3PROTACs faces great challenges.. Here, we report the rational design and evaluation of a series of Gilteritinibbased FLT3-PROTACs.. Among them, B3-2 exhibited the strongest antiproliferative activity against FLT3-ITD mutant AML cells, and significantly induced FLT3-ITD protein degradation.

Immunohistochemistry:

Article Title: Discovery of a potent Gilteritinib-based FLT3-PROTAC degrader for the treatment of Acute myeloid leukemia.
Article Snippet: Fms-like tyrosine receptor kinase 3 (FLT3) proteolysis targeting chimeras (PROTACs) emerge as a promising approach to overcome the limitations of FLT3 inhibitors, while the development of orally bioavailable FLT3PROTACs faces great challenges.. Here, we report the rational design and evaluation of a series of Gilteritinibbased FLT3-PROTACs.. Among them, B3-2 exhibited the strongest antiproliferative activity against FLT3-ITD mutant AML cells, and significantly induced FLT3-ITD protein degradation.

Multiplex Assay:

Article Title: Co-Delivery of aPD-L1 and CD73 Inhibitor Using Calcium Phosphate Nanoparticles for Enhanced Melanoma Immunotherapy with Reduced Toxicity.
Article Snippet: .. Multiplex Immunohistochemical (Multi-IHC) Assay: Tumor tissue slides (3–5 μm) were dewaxed and rehydrated using a gradient alcohol series, followed by antigen retrieval with citrate buffer (Servicebio, pH 6.0). .. For the multi-IHC procedure, protein blocking was performed using a blocking dilution (PerkinElmer).

Immunohistochemical staining:

Article Title: Co-Delivery of aPD-L1 and CD73 Inhibitor Using Calcium Phosphate Nanoparticles for Enhanced Melanoma Immunotherapy with Reduced Toxicity.
Article Snippet: .. Multiplex Immunohistochemical (Multi-IHC) Assay: Tumor tissue slides (3–5 μm) were dewaxed and rehydrated using a gradient alcohol series, followed by antigen retrieval with citrate buffer (Servicebio, pH 6.0). .. For the multi-IHC procedure, protein blocking was performed using a blocking dilution (PerkinElmer).



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